Reduction in bacterial counts in infected root canals after rotary or hand nickel–titanium instrumentation – a clinical study
, , . Reduction in bacterial counts in infected root canals after rotary or hand nickel–titanium instrumentation - a clinical study. International Endodontic Journal International Endodontic Journal, 00, 000–000, 2013.
Abstract
Aim
To
compare the antibacterial efficacy of two instrumentation techniques,
one using hand nickel–titanium (NiTi) instruments and the other using
rotary NiTi instruments, in root canals of teeth with apical
periodontitis.
Methodology
Root
canals from single-rooted teeth were instrumented using either hand
NiTi instruments in the alternated rotation motion technique or rotary
BioRaCe instruments. The irrigant used in both groups was 2.5% NaOCl.
DNA extracts from samples taken before and after instrumentation were
subjected to quantitative analysis by real-time polymerase chain
reaction (qPCR). Qualitative analysis was also performed using
presence/absence data from culture and qPCR assays.
Results
Bacteria
were detected in all S1 samples by both methods. In culture analysis,
45% and 35% of the canals were still positive for bacterial presence
after hand and rotary NiTi instrumentation, respectively (P > 0.05).
Rotary NiTi instrumentation resulted in significantly fewer
qPCR-positive cases (60%) than hand NiTi instrumentation (95%) (P = 0.01). Intergroup comparison of quantitative data showed no significant difference between the two techniques.
Conclusion
There
was no significant difference in bacterial reduction in infected canals
after instrumentation using hand or rotary NiTi instruments. In terms
of incidence of positive results for bacteria, culture also showed no
significant differences between the groups, but the rotary NiTi
instrumentation resulted in more negative results in the more sensitive
qPCR analysis.
Comments