Incorporation of antimicrobial agents can be used to enhance the antibacterial effect of endodontic sealers
Dental Materials
Volume 29, Issue 3 , Pages e29-e34, March 2013
°C).
From the bacteria grown in the liquid medium, the density of the
inoculum was set to be equivalent to McFarland 2 standard. In Shaedler
agar, 350μL of the bacterial suspension were equally spread. Specimens (4mm×6mm)
were prepared from each material without and with addition of 2% BC or
2% CPC. The inhibition zones were determined after 2 days, after 7 days
and after 21 days of incubation.
Volume 29, Issue 3 , Pages e29-e34, March 2013
Abstract
Aim
The antibacterial activity of five endodontic sealers against three different microorganism strains alone and following incorporation of 2% benzalkonium chloride (BC) and 2% cetylpyridinium chloride (CPC) was evaluated.Methodology
The agar diffusion method was used to determine the inhibitory effect of the following endodontic sealers: RoekoSeal, Endomethasone N, N2, Apexit Plus and AH plus, on Streptococcus mutans – ATCC 25175, Lactobacillus casei – ATCC 4646 and Actinomyces viscosus – ATCC 19246. Bacterial strains were inoculated into BHIB, and incubated in an anaerobic atmosphere (37°C).
From the bacteria grown in the liquid medium, the density of the
inoculum was set to be equivalent to McFarland 2 standard. In Shaedler
agar, 350μL of the bacterial suspension were equally spread. Specimens (4mm×6mm)
were prepared from each material without and with addition of 2% BC or
2% CPC. The inhibition zones were determined after 2 days, after 7 days
and after 21 days of incubation.
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