The antibacterial activity of five
endodontic sealers against three different microorganism strains alone
and following incorporation of 2% benzalkonium chloride (BC) and 2%
cetylpyridinium chloride (CPC) was evaluated.
agar diffusion method was used to determine the inhibitory effect of
the following endodontic sealers: RoekoSeal, Endomethasone N, N2, Apexit
Plus and AH plus, on Streptococcus mutans – ATCC 25175, Lactobacillus casei – ATCC 4646 and Actinomyces viscosus – ATCC 19246. Bacterial strains were inoculated into BHIB, and incubated in an anaerobic atmosphere (37°C).
From the bacteria grown in the liquid medium, the density of the
inoculum was set to be equivalent to McFarland 2 standard. In Shaedler
agar, 350μL of the bacterial suspension were equally spread. Specimens (4mm×6mm)
were prepared from each material without and with addition of 2% BC or
2% CPC. The inhibition zones were determined after 2 days, after 7 days
and after 21 days of incubation.
inhibition zones were shown at zero time in all cases, with
progressively less inhibition at 7 and 21 days. Endomethasone N and N2
showed the most intense antimicrobial activity, while RoekoSeal showed
the least antimicrobial effect. The most susceptible microorganism was A. viscosus.
Greater antimicrobial effects were found following incorporation of BC
or CPC, and generally, BC gave greater inhibition zones than CPC.
either BC or CPC has the potential to improve clinical outcomes with
endodontic sealers, as these substances enhance the short-term
antimicrobial effects of the sealers.