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Biodegradation of resin–dentin interfaces is dependent on the restorative material, mode of adhesion, esterase or MMP inhibition

Publication HistoryPublished online: May 19, 2018Accepted: May 8, 2018 Received in revised form: April 2, 2018 Received: August 22, 2017 Abstract Objective To measure the effect of simulated human salivary esterases (SHSE) and metalloproteinases (MMP) inhibition on the integrity of restoration–tooth interfaces made from traditional or polyacid-modified resin composites bonded to human dentin by either total-etch or self-etch adhesives. Methods Resin–dentin specimens, made from traditional (Z250) or polyacid-modified (Dyract-eXtra) composites were bonded to human dentin using total-etch (TE-Scotchbond) or self-etch (SE-EasyBond) adhesives. TE was applied with or without the MMP inhibitor galardin. Specimens were incubated in phosphate-buffer or SHSE (37 °C/pH = 7.0) for up to 180 days, then suspended in a continuous flow biofilm fermenter cultivating biofilms of Streptococcus mutans UA159. Interfacial bacterial penetration, biofilm biomass and viability were measured by conf…

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