easily available tooth storage medium is required to preserve a tooth
after avulsion. Milk and Hank's balanced salt solution (HBSS) are
recommended as tooth storage media, and egg white is also reported to be
comparable with milk. The aim of the present study was to
histologically and immunohistochemically evaluate the effect of
different tooth storage media on the periodontal ligament (PDL) of
Material and Methods
This experiment used HBSS, milk and egg white as tooth storage media. A total of
ninety-six 6-week-old male Sprague-Dawley rats were used in these
experiments. In each experiment, six rats were used for each medium and
for the control group. Extracted rat molar teeth were immersed in these
three different storage media for one-hour. In each medium, six samples
(n=18) were fixed immediately, and the remaining samples (n= 54) were
subcutaneously transplanted. In the control group (n=24), the extracted
teeth were fixed or transplanted immediately after extraction. At day
four, one week and two weeks after transplantation, the teeth were
examined by radiographic, histological and immunohistochemical methods.
The number of PDL cells in the storage media was also counted.
immersed for one-hour in milk showed the thinnest PDL.
Immunohistochemistry of periostin and CD68 labeling suggested
degradation of the extracellular matrix in the PDL. In the media used
for immersion, more PDL cells were observed in milk than in the other
solutions. After transplantation, the HBSS and egg white groups
maintained adequate thickness of PDLbut in the milk group, thinner PDL
and ankylosis were observed.
thickness of PDL was maintained in the egg white group. Whereas, the
milk group showed disturbance in the PDL, which may lead to ankylosis.