In vitro and in vivo investigation of the biological and mechanical behaviour of resin-modified glass-ionomer cement containing chlorhexidine
Available online 30 October 2012
Abstract
Objectives
To
evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical
properties of a resin-modified glass ionomer cement (RMGIC) containing
different concentrations of chlorhexidine (CHX) and (2) the in vivo
microbiologic action of the best concentration of CHX associated with
the RMGIC applied on remaining dentine after indirect pulp treatment
(IPT).
Methods
For
the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5%
CHX. Microbiologic evaluation consisted of an agar diffusion test on
cariogenic bacteria for 24 h. Odontoblast-like cell metabolism and
morphology analyses measured the cytotoxic effects of the RMGIC groups
after 24 h. The same groups were submitted to compressive and diametral
tensile strength. The in vivo treatment consisted of IPT using an RMGIC
associated with the best CHX concentration. Clinical and microbiologic
evaluations were performed before and after 3 months.
Results
The
use of 1.25% CHX significantly improved the antibacterial effects of
the evaluated RMGIC, without causing any detrimental effects to the
odontoblast-like cells and on the mechanical properties. This RMGIC and
CHX combination completely eliminated mutans streptococci after 3 months
of IPT.
Conclusion
The
RMGIC and 1.25% CHX mixture showed great biological and mechanical
behaviour and could be a good treatment against caries progression.
Clinical significance
The association of CHX with a liner RMGIC opens a new perspective for arresting residual caries after IPT.
Comments