Available online 3 September 2018
open access
Abstract
Background/purpose
It is well-known that diverse types of blood proteins
contribute to healing process via different mechanisms. Presence and
potential involvements of blood-derived abundant proteins in the
platelet-rich fibrin
(PRF) to its regenerative capacity have not been sufficiently
emphasized in the literature. The aim of this paper was to analyze the
abundant proteome content of PRF and summarize previously reported effects of identified proteins on wound healing via a literature review.
Materials and methods
The PRF samples obtained from non-smoking, systemically healthy volunteers were subjected to 2D gel electrophoresis
after extracting the proteins from fibrin matrices. All matching spots
were excised from the gels and identified by MALDI TOF/TOF MS/MS analysis. A literature review was conducted to reveal possible contributions of identified proteins to wound healing.
Results
Totally, thirty-five blood proteins were commonly identified among all studied samples. These proteins included serine protease inhibitors, such as alpha-1-antitrypsin, alpha-1-antichymotrypsin, alpha-1-acid glycoprotein, inter-alpha-trypsin-inhibitor, protease C1 inhibitor, and complement proteins. In addition, abundant presence of immunoglobulin G was observed. The abundance of albumin, haptoglobin, ceruloplasmin vitronectin, fetuin-A, ficolin-3 and transthyretin was also detected.
Conclusion
The
results of this study indicated that PRF abundantly contains
blood-origin actors which were previously reported for their direct
contribution to wound healing. Further studies exploring the protein
content of PRF are needed to reveal its undisclosed potential roles in
the healing process.
Comments