Braz. oral res. vol.32 São Paulo 2018
Epub Feb 05, 2018
http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0004
Radiation combined injury, a life-threatening condition, has higher
mortality than simple radiation injury. The aim of the present study was
to analyze the efficiency of
Aloe vera and silver
nanoparticles in improving the healing of ulcerated oral mucosa after
irradiation. Thirty male Albino mice were divided into five groups:
control, radiation, Aloe vera (AV), silver nanoparticles (NS), and
AV+NS. The mice were exposed to whole body 6Gy gamma-radiation. After
one hour, 20% acetic acid was injected into the submucosal layer of the
lower lip for ulcer induction. The animals received topical treatment
with the assigned substances for 5 days. Lip specimens were subjected to
hematoxylin and eosin and anti alpha-smooth muscle actin
immunohistochemical staining. Results demonstrated occurance of ulcer
three days post irradiation in all groups except in the AV+NS group
where only epithelial detachment was developed. After seven days, data
revealed persistent ulcer in radiation group, and almost normal
epithelium in the AV+NS group. A significant reduction of epithelial
thickness was detected in all groups at the third day as compared to
control. At the seventh day, only the AV+NS group restored the
epithelial thickness. Area percent of alpha-smooth muscle actin
expression was significantly decreased in radiation group at the third
day followed by significant increase at the seventh day. However, all
treatment groups showed significant increase in alpha-smooth muscle
actin at the third day, which decreased to normal level at the seventh
day. Our study demonstrated the efficiency of
Aloe vera and silver nanoparticles in enhancing ulcer healing after irradiation.
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