Caries Res 2018;52:331-338
Abstract
This study investigated the remineralization effect of
experimental mint formulations containing bioactive agents (xylitol;
green tea extract, GT; and amorphous calcium phosphate, ACP) in the
progression of artificially induced root caries. Root caries lesions
were induced by demineralization solution (pH 4.6; 96 h; 37°C). The
lesions were treated with mint A, mint B, mint C, xylitol, GT, ACP, or
remineralization solution (RS; negative control). Specimens were
pH-cycled through treatments (5×/day; 3 min) and 6 cycles of acidic (pH
5.0; 30 min) and neutral (pH 7.0; 10 min) buffers for 8 days. Bacterial
collagenase (
Clostridium histolyticum) was used overnight to
simulate proteolytic challenge. Caries depth and porosity as well as
mineral density were estimated using fluorescence microscopy (
n = 15) and microcomputed tomography (
n = 6). Analysis of variance (ANOVA, α = 0.05) showed no statistically significant difference in caries depth among all groups (
p
= 0.172). The highest fluorescence intensity decrease was observed for
GT followed by mint C, with no significant difference between them (
p = 0.868). There were significant differences among GT and mints A, B, and C when compared to RS (
p < 0.001). No statistically significant differences in fluorescence intensity were observed among ACP, xylitol, and RS (
p > 0.05). The mineral density of the lesions in GT, mints A, B, and C, and ACP was statistically similar (
p > 0.05) and significantly higher than that in RS (
p < 0.05). No significant difference was observed between xylitol and RS (
p
= 0.728). The experimental mints showed remineralization action on
artificial root caries, and GT was found to be the main active
ingredient in the investigated formulations.
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