Effect of Er:YAG laser enamel conditioning and moisture on the microleakage of a hydrophilic sealant
Abstract
For
 a given sealant, successful pit and fissure sealing is principally 
governed by the enamel conditioning technique and the presence of 
moisture contamination. A new generation of hydrophilic resin sealants 
is reported to tolerate moisture. This study investigates the impact of 
Er:YAG laser pre-conditioning and moisture contamination on the 
microleakage of a recent hydrophilic sealant. Occlusal surfaces of 
extracted human molars were either acid etched (n = 30), or successively lased and acid etched (n = 30).
 Ten teeth from each group were either air-dried, water-contaminated, or
 saliva-contaminated prior to sealing with UltraSeal XT® 
hydro™. Samples were inspected for penetration of fuchsin dye following 
3000 thermocycles between 5 and 50 °C, and the enamel–sealant interfaces
 were observed by scanning electron microscopy (SEM). Significant 
differences in microleakage were evaluated using the Mann–Whitney U test with Bonferroni adjustment (p = 0.05).
 Laser pre-conditioning significantly reduced dye penetration 
irrespective of whether the enamel surface was moist or dry. 
Microleakage of water-contaminated acid etched teeth was significantly 
greater than that of their air-dried or saliva-contaminated 
counterparts. SEM analysis demonstrated good adaptation in all groups 
with the exception of water-contaminated acid etched teeth which 
exhibited relatively wide gaps. In conclusion, this hydrophilic sealant 
tolerates the presence of saliva, although water was found to impair its
 sealing ability. Laser pre-conditioning significantly decreases 
microleakage in all cases.
Comments