Influence of daily immersion in denture cleanser on multispecies biofilm
Date:
04 Mar 2014
Abstract
Objective
The aim of this study was to evaluate the effect of daily exposure to a denture cleanser on a multispecies biofilm.
Materials and methods
Multispecies biofilms (five bacteria and Candida albicans)
were developed for 64.5 h on acrylic resin specimens and randomized
into control and experimental groups. In the experimental group,
biofilms were immersed in denture cleanser for 3 min/day for seven
consecutive days. In the control group, the biofilms were developed with
no treatment for the same period. Biofilms from both groups were
collected after 1, 4, and 7 days and analyzed for the number of
microorganisms and polysaccharide concentrations. Scanning electron microscopy (SEM) and confocal microscopy (CLSM) analyses were performed.
Results
The
total microorganism counts and bacterial populations were lower in the
experimental group compared to the control group for all of the periods
evaluated. However, the C. albicans counts continuously increased in all of the cleanser-exposed biofilms, with abundant hyphae forms on SEM and CLSM images. The polysaccharide concentration was significantly higher in the experimental group after 7 days.
Conclusions
Daily exposure of a multispecies biofilm to a denture cleanser reduces the number of total microorganisms but favors C. albicans development.
Clinical relevance
Daily use of denture cleanser is an effective method for controlling bacteria in biofilm, but it can potentially select C. albicans, an important etiological agent of oral candidosis.
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