The aim of this study was to determine
the effect of non-surgical periodontal treatment on gingival crevicular
fluid (GCF) and serum oxidant–antioxidant levels in smoking and
non-smoking patients with chronic periodontitis.
patients with chronic periodontitis (15 smokers (CP-S) and 14
non-smokers (CP-NS)) and 20 periodontally healthy subjects (10 smokers
(H-S) and 10 non-smokers (H-NS)) totalling 49 subjects were included in
this study. GCF was collected from at least two pre-selected sites (one
moderate and one deep pocket) in patients with CP. In the healthy group,
GCF samples were collected from one site. Probing pocket depth,
clinical attachment level (CAL), gingival and plaque indices, and
bleeding on probing were measured. To determine serum total oxidant
status (TOS) and total antioxidant status (TAS), venous blood was drawn
from each subject. The GCF, serum sampling, and clinical measurements
were recorded at baseline and 6 weeks after periodontal treatment.
study showed statistically significant improvement of clinical
parameters after periodontal treatment in both smokers and non-smokers.
In the CP-S group, there were no significant differences in GCF TAS
levels at both moderate and deep pocket sites between baseline and 6
weeks (p>0.05). GCF TAS levels in the CP-NS groups were significantly increased (p<0.05)
at moderate and deep pocket sites between baseline and 6 weeks. GCF TOS
levels in the CP-S groups were significantly decreased (p<0.05)
at deep pocket sites between baseline and 6 weeks. There was no
significant difference in serum TAS levels of the all periodontitis
patient groups between at baseline and 6 weeks (p>0.05). Serum TOS levels in the CP-S and CP-NS groups were significantly decreased (p<0.05) after periodontal treatments.
periodontal treatment improves the clinical parameters in both smokers
and non-smokers. These results confirm that non-surgical periodontal
therapy can reduce oxidative stress.