Şifa Güneş, Emrullah Bahsi, Bayram İnce, Hakan Çolak, Mehmet Dalli, İzzet Yavuz, Cafer Sahbaz & Suzan Cangül (2014) Comparative Evaluation of the Effects of Ozone, Diode Laser, and Traditional Cavity Disinfectants on Microleakage, Ozone: Science & Engineering: The Journal of the International Ozone Association, 36:2, 206-211, DOI: 10.1080/01919512.2013.842888
Aim: This study aimed to evaluate the effects on microleakage of the application of ozone gas, laser and tra- ditional cavity disinfection under in vitro conditions.
Material and Methods: Ninety third-molar teeth extracted for various reasons were used in this study. All the teeth were prepared with a standard V cavity on the buccal sur- face. Then the teeth were randomly allocated to one of 6 groups of 15 teeth; Group 1 benzalkonium chloride, Group 2 chlorhexidine gluconate, Group 3 sodium hypochloride, Group 4 diode laser, Group 5 ozone gas, Group 6 control group- no disinfection was applied. Primer was applied to the cavities, then bond and 10-s polymerization. Clearfil AP-X was used in the cavities as a hybrid composite and poly- merized for 20 s. The samples then underwent 1000 thermal cycles of 30-s application, in baths at temperatures between 5 ± 2 ◦C and 55 ± 2 ◦C. All the samples were stirred in 0.5% basic phuxine solution and the sections taken under stereomicroscope were examined and photographed at 15 × magnification. SEM analysis was made and the obtained results were statistically evaluated with the Kruskal–Wallis test.
Results: No significant difference was seen between the groups in respect of the microleakage values of both the occlusal and gingival edges (p > 0.05). Comparison of the microleakage values of the occlusal and gingival edges of the groups determined the least leakage to be in the ozone group (p > 0.05).
Conclusion: No statistical significance was determined between the groups. However, the mean least microleakage was found in the ozone group and the highest amount in the control group.