Friday, November 21, 2014

Influence of daily immersion in denture cleanser on multispecies biofilm

, Volume 18, Issue 9, pp 2179-2185
Date: 04 Mar 2014



The aim of this study was to evaluate the effect of daily exposure to a denture cleanser on a multispecies biofilm.

Materials and methods

Multispecies biofilms (five bacteria and Candida albicans) were developed for 64.5 h on acrylic resin specimens and randomized into control and experimental groups. In the experimental group, biofilms were immersed in denture cleanser for 3 min/day for seven consecutive days. In the control group, the biofilms were developed with no treatment for the same period. Biofilms from both groups were collected after 1, 4, and 7 days and analyzed for the number of microorganisms and polysaccharide concentrations. Scanning electron microscopy (SEM) and confocal microscopy (CLSM) analyses were performed.


The total microorganism counts and bacterial populations were lower in the experimental group compared to the control group for all of the periods evaluated. However, the C. albicans counts continuously increased in all of the cleanser-exposed biofilms, with abundant hyphae forms on SEM and CLSM images. The polysaccharide concentration was significantly higher in the experimental group after 7 days.


Daily exposure of a multispecies biofilm to a denture cleanser reduces the number of total microorganisms but favors C. albicans development.

Clinical relevance

Daily use of denture cleanser is an effective method for controlling bacteria in biofilm, but it can potentially select C. albicans, an important etiological agent of oral candidosis.

Thursday, November 20, 2014

Six-month results following treatment of aggressive periodontitis with antimicrobial photodynamic therapy or amoxicillin and metronidazole

, Volume 18, Issue 9, pp 2129-2135
Date: 04 Feb 2014



The use of antibacterial photodynamic therapy (aPDT) additionally to scaling and root planing (SRP) has been shown to positively influence the clinical outcomes. However, at present, it is unknown to what extent aPDT may represent a potential alternative to the use of systemic antibiotics in nonsurgical periodontal therapy in patients with aggressive periodontitis (AP). The aim of this study was to evaluate the outcomes following nonsurgical periodontal therapy and additional use of either aPDT or amoxicillin and metronidazole (AB) in patients with AP.

Material and methods

Thirty-six patients with AP displaying at least three sites with pocket depth (PD) ≥6 mm were treated with SRP and either systemic administration of AB for 7 days or with two episodes of aPDT. The following clinical parameters were evaluated at baseline and at 6 months: plaque index (PI), bleeding on probing (BOP), PD, gingival recession (GR) and clinical attachment level (CAL).


Thirty-five patients have completed the 6-month evaluation. At 6 months, mean PD was statistically significantly reduced in both groups (from 5.0 ± 0.8 to 3.0 ± 0.6 mm with AB and from 5.1 ± 0.5 to 3.9 ± 0.8 mm with aPDT (p < 0.001)). AB yielded statistically significantly higher improvements in the primary outcome parameter PD (p < 0.001) when compared to aPDT. The number of pockets ≥7 mm was reduced from 141 to 3 after AB (p < 0.001) and from 137 to 45 after aPDT (p = 0.03). Both therapies resulted in statistically significant reductions in all parameters compared to baseline.


While both treatments resulted in statistically significant clinical improvements, AB showed statistically significantly higher PD reduction and lower number of pockets ≥7 mm compared to aPDT.

Clinical relevance

In patients with AP, the two times application of aPDT in conjunction with nonsurgical periodontal therapy cannot be considered an alternative to the systemic use of amoxicillin and metronidazole.

Wednesday, November 19, 2014

Microbial contamination of laboratory constructed removable orthodontic appliances

, Volume 18, Issue 9, pp 2193-2202



This study aims to determine whether laboratory constructed removable orthodontic appliances are free from microbial contamination prior to clinical use and to evaluate the dental hospital cross-infection procedures to ensure that patient-derived contamination does not enter the construction process, thereby propagating a cycle of cross-contamination.

Materials and methods

The construction process of removable orthodontic appliances from three individuals was evaluated at every stage, from impression to final delivery of the appliance using molecular microbiological techniques. The bacterial profiles at each stage of appliance construction were obtained using denaturing gradient gel electrophoresis, along with the bacterial profiles of the three participants’ saliva. This enabled the bacterial profiles found at each stage of construction to be compared directly with the saliva of the person for whom the appliance was being constructed. Bacteria were identified at each stage using 16S rDNA PCR amplification and sequence phylogeny.


There was no evidence of bacterial cross-contamination from patients to the laboratory. The current process of disinfection of impression appears to be adequate. Contamination was found on the final removable appliances (0.97 × 102–1.52 × 103 cfu ml−1), and this contamination occurred from within the laboratory itself.


Every effort is made to reduce potential cross-infection to patients and dental professionals. Newly constructed removable appliances were shown not to be free from contamination with bacteria prior to clinical use, but this contamination is environmental. Further studies would be required to determine the level of risk this poses to patients.

Clinical significance

Dental professionals have a duty of care to minimise or eradicate potential risks of cross-infection to patients and other members of the team. To date, much less attention has been paid to contamination from the orthodontic laboratory, so contamination and infection risks are unknown.

Tuesday, November 18, 2014

Effect of periodontal treatment on peak serum levels of inflammatory markers

, Volume 18, Issue 9, pp 2113-2121


Background and objective

Some subjects with untreated periodontitis exhibit elevated levels of distinct inflammatory markers in serum. The aim of the study was to assess whether nonsurgical periodontal therapy changes the levels of these markers and lowers these peaks.


Forty periodontally diseased subjects received nonsurgical periodontal therapy (full-mouth scaling and root planing within 48 h) with either adjunctive systemic amoxicillin and metronidazole (n = 19) or placebo (n  = 21). Serum samples, obtained at baseline (BL) and 3 months after treatment (M3), were evaluated for 15 cytokines and 9 acute-phase proteins using the Bio-Plex bead array multianalyte detection system. For each analyte, peak values were defined as greater than the mean + 2 standard deviations (SD) of measurements found in 40 periodontally healthy persons. Proportions were compared using Fisher’s exact test.


At M3, a significantly better primary clinical outcome (persisting pockets of >4 mm with bleeding on probing) was obtained in patients treated with scaling and root planing plus antibiotics compared to those receiving placebo (3.3 ± 5.1 vs. 6.8 ± 7.8 pockets per patient, p  < 0.05). The levels of cytokines and acute-phase proteins of periodontitis patients were usually below the mean + 2 SD threshold of healthy persons. However, values above threshold were found in some individuals. Eleven patients showed a peak value of one analyte, and seven patients showed two peaks. In the remaining 12 patients, between three and ten analytes showed peak values. Therapy greatly reduced the number of subjects with four or more peaks (BL, 11 subjects; M3, 1 subject, p = 0.003). With regards to the reduction of peaks, no specific benefit of adjunctive antibiotics could be seen.


Subjects with untreated periodontitis may show high peaks for several inflammatory markers in serum simultaneously. Nonsurgical periodontal treatment with or without antibiotics reduced most of these peak levels.

One-sentence summary

Nonsurgical periodontal treatment with or without antibiotics reduced peak levels of several inflammatory markers in serum of subjects with periodontitis.

Monday, November 17, 2014

New Web Tool Provides Immediate Repair Requests, Online Education and Training for All StarDental® Customers

DentalEZ® Group Introduces the 360° Customer Care Program
Malvern, PA (November 13, 2014) – DentalEZ® Group, a supplier of integrated products and services for dental health professionals worldwide, is pleased to introduce its new 360° Customer Care Program, a comprehensive online customer service tool for all StarDental® customers.

Located at, the new 360° Customer Care Program is an extension of DentalEZ Group’s professional and preventive maintenance and repair support program. Geared toward all dental professionals and distribution partners, the 360° Customer Care Program provides a newly designed repair support program that provides instantaneous labels for repair requests as well as a comprehensive collection of educational and training resources for all StarDental products.   

A key feature of the 360° Customer Care Program is a new repair request form that provides an efficient, no-hassle method of requesting repairs on any and all StarDental products. Designed for a fast, user-friendly experience, the repair request form provides convenient drop down menus for identifying and immediately reporting the specific product, repair type and problem. Users simply fill in the information, print the repair request form and press the submit button. A prepaid UPS label is available to be shipped along with the repair form and product being sent for repair.

In addition to the new repair request form, visitors of the 360° Customer Care Program have access to the following pertaining to StarDental’s full line of products:

·       Maintenance and repair videos
·       Instructional manuals
·       Product brochures
·       Maintenance check sheets
·       Warranty registration forms

For more information on DentalEZ’s new 360° Customer Care Program, please visit

About DentalEZ® Group

DentalEZ® Group is committed to advancing the practice of dentistry through innovative products and services. Encompassing six distinct product brands — StarDental®, DentalEZ®, CustomAir®, RAMVAC®, NevinLabs and Columbia Dentoform® — DentalEZ® Group manufactures everything in the operatory, from handpieces to chairs to vacuum systems to dental simulation models, creating a complete line of products to elevate the health, comfort
and efficiency of the dental operatory. For more information, please visit

Friday, November 14, 2014

Intra-oral Adjustment/Polishing for Zirconia and Lithium Disilicate

  Garrison Dental Solutions  
INTRODUCING a NEW product from Garrison!

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Intra-oral Adjustment/Polishing for Zirconia and Lithium Disilicate
eZr™ is a three-step diamond impregnated polishing system that polishes zirconia and e-max®† to smooth.
  • Enables easy and safe adjustment for zirconia and lithium disilicate.
  • Proprietary diamond impregnated polisher reduces possibility of chipping and microfracturing.
  • Provides an unparalleled smoothly polished occlusal surface, eliminating abrasiveness to opposing dentition.
  • Prevents over-heating that could lead to premature failure.
  • Eliminates the need for polishing paste, reducing overall cost per procedure.
† Not a product or trademark of Garrison Dental Solutions, LLC.
Complete system kit, 2 grinders and 6 polishers

Thursday, November 13, 2014

Comparison of microtensile bond strength of a resin composite to enamel conditioned by acid etching and Er,Cr:YSGG laser in human primary teeth




This study was designed to compare the bond strength of composite resin restorations on the buccal surface of primary human canine after conditioning by conventional acid etching and Er,Cr:YSGG laser.


Twenty sound primary canines were cut buccolingually into two halves and each half was randomly placed in Er,Cr:YSGG laser or acid etch group. The samples in the acid etch group were etched with 37 % phosphoric acid for 30 s. The samples in the laser group were prepared by Er,Cr:YSGG laser. The G6-Tips and 600 µm diameter were used with a 1.5 W of power output, pulse duration of 140 µs and repetition rate of 20 Hz. The bonding agent was applied on the buccal surface of each sample and layers of resin composite were placed. The samples’ bond strengths were evaluated by a microtensile test instrument.


The mean of microtensile bond strength was 18.55 ± 6.41 in the laser group and 24.62 ± 5.56 in acid etch group. Microtensile bond strength achieved by laser conditioning was significantly lower than microtensile bond strength achieved by the conventional acid etching. Statistics To compare the results between the acid etch and laser group, the paired t test was performed (p value < 0.001).


Conditioning enamel in primary teeth by Er,Cr:YSGG laser, cannot be used as an alternative method for acid etching and cannot substitute this conventional method.