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Thursday, June 04, 2015
Influence of phosphoproteins’ biomimetic analogs on remineralization of mineral-depleted resin–dentin interfaces created with ion-releasing resin-based systems
April 22, 2015
study aimed at evaluating the remineralization of acid-etched dentin
pre-treated with primers containing biomimetic analogs and bonded using
an ion-releasing light-curable resin-based material.
An experimental etch-and-rinse adhesive system filled with Ca2+, PO43−-releasing
Ca-Silicate micro-fillers was created along with two experimental
primers containing biomimetic analogs such as sodium trimetaphosphate
(TMP) and/or polyaspartic acid (PLA). Dentin specimens etched with 37% H3PO4
were pre-treated with two different aqueous primers containing the
polyanionic biomimetic analogs or deionized water and subsequently
bonded using the experimental resin-based materials. The specimens were
sectioned and analyzed by AFM/nanoindentation to evaluate changes in the
modulus of elasticity (Ei) across the resin–dentin interface at
different AS storage periods (up to 90 days). Raman cluster analysis was
also performed to evaluate the chemical changes along the interface.
The phosphate uptake by the acid-etched dentin was evaluated using the
ATR-FTIR. Additional resin–dentin specimens were tested for microtensile
bond strength. SEM examination was performed after de-bonding, while
confocal laser microscopy was used to evaluate the interfaces
ultramorphology and micropermeability.
biomimetic primers induced phosphate uptake by acid-etched dentin.
Specimens created with the ion-releasing resin in combination with the
pre-treatment primers containing either PLA and TMA showed the greatest
recovery of the Ei of the hybrid layer, with no decrease in μTBS (p
> 0.05) after 3-month AS storage. The ion-releasing resin applied
after use of the biomimetic primers showed the greatest reduction in
micropermeability due to mineral precipitation; these results were
confirmed using SEM.
use of the ion-releasing resin-based system applied to acid-etched
dentin pre-treated with biomimetic primers containing analogs of
phosphoproteins such as poly-l-aspartic acid
and/or sodium trimetaphosphate provides a suitable bonding approach for
biomimetic remineralization of resin–dentin interfaces.